Medicago Sequencing

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Genome assembly release: January, 2008: The current release 2.0 assembly was finalized on 8/10/2007, using BACs frozen on 6/15/2007. The assembly was released in January 2008.; Mt2.0 release — The current Medicago genome assembly release site.; Mt1.0 release — Medicago genome assembly version 1.0.
BAC sequences: June 28, 2008: These files contain all sequenced BACs, and are updated twice weekly.; Check the readme for file date and unzipped file size: MtBACs_readme.txt; MtBACsPh1.tar.gz — Phase 1 BACs: shotgun; multiple pieces, not ordered or oriented.; MtBACsPh2.tar.gz — Phase 2 BACs: multiple pieces, but ordered and oriented.; MtBACsPh3.tar.gz — Phase 3 BACs: finished sequence.; BACsNotInMt1.tar.gz — New BACs sequenced after Release Mt1.0. (BACsNotInMt1.0_11062007.txt)

Gene predictions: February, 2008: Gene predictions on finished BACs, from the International Medicago Genome Annotation Group (IMGAG).; Check the IMGAG process, parameters, and file syntax at the: description site . Files are also available at MIPS-UrMeLDB.; 20080227_imgag_cds.fa.tar.gz — coding sequences of all predicted gene models on individual BACs.; 20080227_imgag_prot.fa.tar.gz —protein sequences of all predicted gene models on individual BACs.

Marker Report: June 2007: markerreport_20070607_modified.xls — A report on all markers: marker location, the associated BACs, FPC, primers/full squences.

BAC-end Sequences: October 18, 2005: Check the readme file for BAC-end sequence information: Mt_BES_readme.txt; Mt_BES_mth2.fas.gz — All BAC end sequences from the Hind III library; Mt_BES_mte1.fas.gz — All BAC end sequences from the French Eco RI library; Mt_BES_mth4.fas.gz — BAC ends from a small supplemental Hind III library; Mt_BES_mth4_new.fas.gz — new BAC ends from a small supplemental Hind III library with large inserts (up to more than 200kb)